Data/hora: 22/05/2024 05:44:28

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	27/01/2023	
Data da última atualização: 	24/03/2023	
Autoria: 	MACHADO-ROSA, T. A.; BARBOSA, E. T.; CÔRTES, M. V. de C. B.; LOBO JUNIOR, M.	
Afiliação: 	TARIANE A. MACHADO-ROSA, UNIVERSIDADE FEDERAL DE GOIÁS; ELDER TADEU BARBOSA, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MURILLO LOBO JUNIOR, CNPAF.	
Título: 	Microtiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Rhizosphere, v. 25, 100666, 2023.	
ISSN: 	2452-2198	
DOI: 	https://doi.org/10.1016/j.rhisph.2023.100666	
Idioma: 	Inglês	
Conteúdo: 	A quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 &#956;L of the super-natant were added to 100 &#956;L of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method.	
Palavras-Chave: 	Biocontrol; Plant growth promotion; Stepwise screening.	
Thesagro: 	Estimulante de Crescimento Vegetal; Fungo; Trichoderma.	
Thesaurus Nal: 	Auxins; Plant growth; Root exudates.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (36628-1) - UPC - AP - DD - 2023 -  - 2023 - DIS